The following experiment is done to validate safety of Longlive xylo-oligosaccharides:
Xylo-oligosaccharide Toxicity Test with ICR Mice
Tested material: xylo-oligosaccharides; Tested animal: 6-week-age ICR mice
Test duration: 100d           Test groups: reference group and test group
Test method: feeding mice with water (for reference group) and 3.0g/kg BW xylo-oligosaccharides (test group) from mouth respectively
Safety assessment:  Survival rate; clinical observation;                    
Weight and feedstuff consumption;
Serological examination of liver and renal functions;
Organ weight, etc.
Test result: 100% survived, without any symptom of clinical toxicity shown in test period. Changes in weight and food intake show that weight and food intake of male mice taking xylo-oligosaccharides increases more obviously than the reference group, while there is no difference between female mice of two groups. After the experiment, no abnormity is found upon autopsy and visual observation; serological examination also shows no adverse effect of xylo-oligosaccharides on liver and renal functions. Organ weighing result shows that, feeding xylo-oligosaccharides may obviously increase cecum weight of both male and female mice, while weight of other organs does not differ evidently. For tested young mice, no toxicity is shown after feeding 3.0 g/kg BW xylo-oligosaccharides per day.
Therefore, Longlive xylo-oligosaccharides is safe.